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Ultra-Fast
High Temperature Liquid Chromatography
 Chromatographers
have long known that an increase in operating temperature can dramatically
improve both the efficiency and speed of an HPLC separation. There are three
main effects that happen at elevated temperature. Firstly, the viscosity of the
mobile phase is decreased, so that higher flow rates are possible with existing
equipment without increasing backpressure. Secondly, at higher temperature, the
diffusion of analytes into and back out of the pores of a chromatographic
material is faster, so the efficiency of the separation generally improves.
Finally, at elevated temperature, the kinetics of the interactions between the
analytes and the stationary phase are faster. This will lower the overall
retention, but also often reduces or eliminates tailed peaks.
High Temperature Links: Improving
Throughput with High Temperature Presentation (PDF) E-Newsletter
Vol 1 - "Using Elevated Temperature to Improve HPLC Separations", May 2002
E-Newsletter
Vol 5 -
"High Temperature - The Future of HPLC!", May 2003
High
Throughput - High Temperature LC/ Electrospray-MS Poster (PDF)
(Poster Courtesy of LCMS
Limited, PO Box 27228, Raleigh, NC, USA 27611)
Reducing Run Time with Elevated Temperature
High
Temperature Applications: 
| PDF |
Column(s) |
Compound
Class |
Details |
| 235 |
DB-C18 |
Parabens and Phenols |
Uracil,
Phenol, Methyl Paraben, Ethyl Paraben, Propyl Paraben, Butyl Paraben,
Heptyl Paraben |
| 259 |
PBD |
Antihistamines |
Doxylamine,
Methapyrilene, Chlorpheniramine, Triprolidine, Meclizine; Use of
High Temperature |
| 260 |
DB-C18 |
Barbiturates |
Barbital,
Butabarbital, Pentobarbital, Carbromal, Secobarbital, Methohexital |
| 261 |
PBD |
Benzodiazepines |
7-aminoflunitrazepam,
Clonazepam, Alprazolam, Nordiazepam, Diazepam, Medazepam |
| 266 |
CARB |
Steroids/structurally
similar |
Dihydroestradiol Sulfate,
Dihydroequilin Sulfate, Equilin Sulfate, Estrone Sulfate, Equilenin
Sulfate, Dihydroequilenin Sulfate |
| 269 |
PBD |
Antihistamines |
Doxylamine,
Methapyrilene, Chlorpheniramine, Triprolidine, Meclizine; Use of
High Temperature |
| 271 |
PS |
PAHs |
Napthalene,
1-Methylnapthalene, Acenapthylene, Acenapthene,
Fluorene, Decafluorobiphenyl, Phenanthrene,
Anthracene, Fluoranthene, Pyrene, p-terphenyl-d14,
benzo(a)anthracene, benzo(b)fluoranthene, Benzo(a)pyrene,
Dibenzo(a,h)anthracene, Benzo(g,h,i)perylene |
| 276 |
PBD |
Antihypoglycemic |
Glyburide |
| 279 |
DB-C18 |
Food/Cosmetics |
Nitrosamines |
| 284 |
DB-C18 |
Steroids |
Epietiocholanolone,
Etiocholanolone, Androsterone, Epiandrosterone |
NOW
AVAILABLE - Short ZirChrom Columns for UFHTLC ZirChrom
now offers short (2 cm) all-stainless steel pre-columns for ultra-fast high
temperature reversed-phase liquid chromatography. These columns are only
sold in sets of two. Mix and match ZirChrom chemistries if you like!
Contact ZirChrom technical support at 1-866-STABLE-1 for more details, or feel
free to contact us online.
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