Volume 12:  

Phosphopeptide Enrichment

In this special edition of Z-APPS we have included six new application notes.  This research highlights the unparalleled selectivity of our titanium dioxide and zirconium dioxide particles (in bulk or in Glygen Lab-in-a-tip SPE tips) for phosphopeptide enrichment. 

Technical Bulletin #322Phosphopeptide Enrichment using Sachtopore®-NP Titanium Dioxide – In this application note we report a rapid, highly selective enrichment procedure utilizing 2,5-dihydroxybenzoic acid (DHB) to enhance the selective enrichment of phosphorylated peptides on a titanium dioxide micro-column. This unique technique dramatically increases the selectivity, and thus sensitivity, of enrichment purification of phosphorylated peptides from complex mixtures of non-phosphorylated and phosphorylated peptides. (PDF - Full Application Note)

Technical Bulletin #323 Phosphopeptide Enrichment Using Titanium Dioxide & Zirconium Dioxide SPE Tips This application note reports a rapid, highly selective enrichment procedure for phosphopeptides utilizing titanium dioxide (TiO2) & zirconium dioxide (ZrO2) SPE tips.  β-casein digest samples purified via NuTip™ SPE tips, manufactured by Glygen Corporation ( Columbia , MD ), display exceptional signal to noise ratios for phosphopeptide analysis and eliminate many difficulties present in traditional IMAC methods. (PDF - Full Application Note)

Technical Bulletin #324 Effect of Loading Buffer on Phosphopeptide Enrichment using Zirconium Dioxide SPE Tips An investigation of the effect of sample loading buffer on a rapid, highly selective enrichment procedure for phosphopeptides utilizing zirconium dioxide (ZrO2) SPE tips.  For α-casein digest samples enriched using ZrO2 NuTip™ SPE tips, manufactured by Glygen Corporation (Columbia, MD), a low pH formic acid loading buffer enabled the most effective and specific enrichment of phosphopeptides. (PDF - Full Application Note)

Technical Bulletin #325Comparison of Titanium Dioxide & Zirconium Dioxide SPE Tips for Phosphopeptide Enrichment A critical comparison of zirconium dioxide (ZrO2) and titanium dioxide (TiO2) SPE tips for rapid enrichment of phosphopeptides.  Although, for the α-casein digest samples tested, either technique proves more effective than traditional methods, interestingly, the ZrO2 tips enriched singly phosphorylated peptides in greater abundance. (PDF - Full Application Note)

Technical Bulletin #326 Selectivity of Titanium Dioxide & Zirconium Dioxide SPE Tips for Phosphopeptide Enrichment This application note compares and contrasts the selectivity of zirconium dioxide (ZrO2) and titanium dioxide (TiO2) SPE tips with varying concentrations of α-casein for rapid enrichment of phosphopeptides.  The results demonstrate that phosphopeptide selectivity for α-casein was not compromised when sample amount was decreased to 25 pmol.  Below 25 pmol, a smaller SPE tip (25 μg) is required to obtain acceptable signal to noise ratios. (PDF - Full Application Note)

To place an order for Glygen’s Lab-in-a-tip SPE tips, or for more information, please contact us at 1-866-STABLE-1 or visit http://www.zirchrom.com/glygen.asp.

 

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